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Stationary-phase Salmonella Typhimurium were opsonized with 8% (v/v) mouse serum in RPMI 1640 for 20 min and used to infect immortalized BMDMs at MOI 15 (ref

Hietbrink F, Besselink MG, Renooij W, et al

Thiol compounds often exhibit distinct absorbance or fluorescence spectra, allowing for their selective detection and quantification in complex mixtures

Recently, a significant role for GSTO1 in protein deglutathionylation has been described 11 , and -actin was identified as one of the cellular substrates for GSTO1-catalysed deglutathionylation

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Some researchers may require higher doses or longer cycles to achieve noticeable effects, though increasing beyond standard protocols should be approached cautiously