A decrease in activity compared to the one of the free enzyme is due to the following reasons: (1) use of organic solvent for the immobilization procedure, which deactivates some parts of enzyme
Mild nausea within the first few days of a dose increase is common and usually resolves within a week as your body adapts
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doi: 10.1111/j.1365-2036.1996.tb00183.x 36 SinghNSinghPNHershmanJM
Many patients continue on a lower maintenance dose, combined with diet and lifestyle changes, to sustain results
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