The cooperative upregulation of glutathione redox system of the proline group should help to more effectively eliminate ROS in the mitochondria, relative to control group
Then, the trypsin/EDTA solution was neutralized with Dulbeccos modified Eagles medium (DMEM, #22320-022, ThermoFisher Scientific, Waltham, MA, USA), supplemented with 10% (v/v) fetal bovine serum (FBS, #S181C, Biowest, Nuaill, France)
In addition, pancaspase inhibitors, which are highly effective against TNF- or Fas receptor-induced apoptosis [43, 44], did not protect [42]
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Gradual dose escalation over weeks to months is essential