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Moreover, key factors determining the measured response to oxidative stress are likely to be exposure time and stress intensity

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Third, the data of Figure 4 show fold-changes compared to wild-type: even if strongly induced at the protein level, low abundance enzymes may not make a marked contribution to GSH oxidation within the cellular context

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The click reaction cocktail was prepared using a Click-iT EdU Imaging kit (Invitrogen, C10337) according to the manufacturers protocol