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In the recording chamber, a single frontal cortex slice was submerged and perfused continuously (2.0 ml/min) with ACSF containing 124 mM NaCl, 3 mM KCl, 2 mM CaCl 2 , 2 mM MgCl 2 , 1 mM NaH 2 PO 3 , 26 mM NaHCO 3 , and 10 mM glucose equilibrated with 95% O 2 and 5% CO 2 , pH 7.357.45

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In the rabbit, higher doses of empagliflozin resulted in maternal and fetal toxicity at 700 mg/kg/day, or 139-times the 25 mg maximum clinical dose