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Complementing these classical antioxidant systems, cancer cells also exploit radical-trapping antioxidant mechanisms mediated by FSP1 and GCH1, both of which are frequently upregulated in diverse malignancies and markedly contribute to ferroptosis resistance (21,139)

Continuous cell viability measurements In total, 10,000 cells (HeLa or U2OS) per well, in DMEM with 2% FCS and without phenol red, were seeded into black 96-well plates with a transparent bottom (655090, Greiner)

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