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So statements that topical AHK-Cu is "well tolerated" rest on the copper-peptide class generally much of it built on GHK-Cu (Pickart & Margolina 2018, PMID 29986520) and on community use, rather than on dedicated AHK-Cu safety study

Half of the samples were also incubated in 4 l of 10 mM TCEP (Sigma-Aldrich) at room temperature for 30 min, to reduce oxidized thiols