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The parental MCF-7 cells and HEK293T17 cells were cultured in DMEM (Gibco, California, USA) with 5% FBS (Excell, Suzhou, China) supplemented with 100 units/mL penicillin and 100 g/mL streptomycin (Beyotime Biotechnology, Jiangsu, China)

The study by Neelakantan et al

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Includes 30 caplets for 30 daily servings formulated for nursing moms

To account for this, the repeated measures approach controlled for individual variation in our analyses