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Separation was done using a C 18 column with mobile phase of acetonitrile and water acidified with orthophosphoric acid (pH 3.5) (1.41 10 5 M) in isocratic mode in ratio 57:43 and 1 mL/min flow rate after extraction using protein precipitation

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Int J Biol Sci 17(8):20502068 Bonfioli GB, Rodella L, Metra M, Vizzardi E (2024) GLP-1 receptor agonists as promising anti-inflammatory agents in heart failure with preserved ejection fraction