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The resin was washed by ten volumes of wash buffer (50 mM Tris-HCl, pH 8.0, 400 mM NaCl, 1 mM CaCl 2 , 5% glycerol, 0.05% DDM) and mIGF1R protein was eluted by HRV-3C protease cleavage

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Glucose starvation inhibits the ability of leptin to stimulate tyrosyl phosphorylation and inhibits the activation of JAK2 and STAT3 in vitro (Su et al., 2012)