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Samples were digested overnight at 37 C in 50 l of 50 mM ammonium bicarbonate, shaking the plate at 1,650 rpm with trypsin and LysC at a 1:50 and 1:100 ratio, respectively

L-theanine supports relaxation and mood by increasing alpha-wave activity, and levels of serotonin, dopamine, and GABA in the brain.* L-theanine also has been shown to block binding of L-glutamic acid to excitatory glutamate receptors in the brain.* Glutamate receptor subtypes have been shown to modulate excitability associated with anxiety, while in depression, a hyperfunctioning glutaminergic system also may be an issue.* Continuous administration of L-theanine has been shown to increase expression of brain-derived neurotrophic factor (BDNF) protein, a protein which plays a role in neural plasticity and neurogenesis.* *These statements have not been evaluated by the Food and Drug Administration

FGF-21 is an endocrine hormone that has been shown to protect against hepatic steatosis, inflammation, and fibrosis in animal models of AUD [135,136]