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2.9 Assessment of apoptosis by flow cytometry Cells were seeded in cRPMI into 12-well plates at optimised seeding densities (HCT116: 400,000 cells/well, SW837: 500,000 cells/well) and allowed to adhere overnight at 37C in 5% CO 2 /95% humidified air

Daily sunscreen helps protect the results

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Using the wrong solvent volume

However, as explained in detail in a recent review by Rae et al

After overnight TEV digestion at 4 C, the GST elution was then loaded on a Superdex 75 Increased 10/300 GL column (Cytiva) preequilibrated with wash buffer