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A drop of 150 nl enzyme/cofactor/substrate solution was mixed with 150 nl precipitant (100 mM MES, pH 6.4, 200 mM MgCl 2 , 17.9% PEG200) and equilibrated against 50 l reservoir solution (100 mM MES, pH 6.4, 200 mM MgCl 2 , 17.9% PEG200)

Glutathione lowers oxidative stress and combats free radicals by increasing antioxidant levels

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