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Its important to know the safe administration guidelines

Vasavada A, Patel P, Sanghavi DK

Vitamin B12 deficiency has been linked to fatigue, tingling or loss of feeling in the extremities, irritability, memory impairment, depression, anemia, irritable bowel syndrome (IBS), constipation, poor wound healing, dry or damaged looking skin, and neurological disorders

They usually start with high doses and then lower them for maintenance

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All mice received 2 days of 10 mL/kg body weight sterile saline subcutaneous injections before beginning the treatment phase to allow for acclimation to the handling and stress associated with injections