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Liraglutide Inhibits the Apoptosis of Human Nucleus Pulposus Cells Induced by High Glucose through PI3K/Akt/caspase-3 Signaling Pathway
Quantitative real-time polymerase chain reaction (qPCR) was performed with TaqMan Gene Expression Assays, according to the manufacturers protocol using TaqMan primers for UCP1 (Hs00222453_m1), UCP2 (Hs01075227_m1), NAMPT (Hs00237184_m1), and FNDC5 (Hs00401006_m1) (Applied Biosystems, Carlsbad, CA, USA)
It is currently unclear whether TIGIT/CD155 inhibitors exert their effects by directly impacting mitochondrial function or through indirect metabolic pathways [94]
The reactions were carried out using phosphate buffer pH 7.8 with 50-fold derivatization reagent excess
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8 , e1114 (2023)
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