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While the available data is promising, further investigation is necessary to comprehensively understand the long-term effects of TQ use in treating skin diseases, including AtD (85, 106)

The Ni-NTA resin was packed into a glass column and washed with 25 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, followed by elution using the same buffer supplemented with 250 mM imidazole and 3 mM CaCl 2

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10.1007/s10495-015-1160-y 84 RizviF.MathurA.KrishnaS.SiddiqiM

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