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PMCID: PMC11258867

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Although the P-value of cholic acid was less than 0.05, there presented a trend of difference between groups

The hair loss skin patches continued to increase in size, with the crown patch measuring 4 4 cm (Fig 1), the occipital patch measuring 3 4 cm (Fig 2), the temporal patch at 2 1 cm (Fig 3), and the frontal patch at 2 2 cm (Fig 4)

After washing, cells were seeded on collagen-coated plates and cultured in attachment medium in 6-well plates under humidity conditions in 95% air and 5% CO 2 at 37 C for 24 h before treatment