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Avoid simultaneous use with strong acids or chelators (e.g., vitamin C or retinol serums) because they can inactivate copper peptides

Some other prescription medications can directly cause a vitamin B12 deficiency

Vitamin B12 is water-soluble but stored in the liver, allowing high serum levels to persist after large supplemental doses

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A 500mcg daily protocol under split dosing would look like: 300mcg upon waking (fasted), then 200mcg approximately 30 minutes before dinner or before bed on an empty stomach