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[DOI] [PubMed] [Google Scholar] [78].Johnson PJ, Casteel SW, Messer NT

If that sentence surprised you, you are not aloneand that reaction is exactly the problem

The signal at 600 nm reflects cysteine content, not total soluble thiol content, not glutathione content, and not the combined reducing power of every small-molecule antioxidant in a crude leaf homogenate