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Progress in Brain Research, 185 , 105-129

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To investigate the effect of gene knockout on l-cysteine and GSH degradation, the engineered strains were cultured in the flasks as described above and the cells were collected by centrifugation (6000 rpm, 4 C for 5 min) after two washes with 50 mM phosphate buffer (pH7.0)

Gen Comp Endocrinol 252:150161 Shimizu M, Kishimoto K, Yamaguchi T, Nakano Y, Hara A, Dickhoff WW (2011a) Circulating salmon 28- and 22-kDa insulin-like growth factor binding proteins (IGFBPs) are co-orthologs of IGFBP-1