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Enzymatic assays To assay the activities of Cp1A and MfaA, 100-l reactions were performed at 30 C for 3 h in 50 mM sodium phosphate buffer (pH 8.0) containing 0.2 mM FeSO 4 , 2 mM KG, 2 mM ascorbate, 1 mM substrate and 10 M Cp1A or MfaA

doi: 10.1016/j.neubiorev.2022.104642 3

Zepbound/Mounjaro is contraindicated for individuals with a personal or family history of medullary thyroid carcinoma (MTC) or Multiple Endocrine Neoplasia syndrome type 2 (MEN 2)

This enzyme is called glutathione reductase (GSR) (Ganea & Harding, 2006)