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For example, stromal cell-derived factor 1 alpha (SDF-1) doubled the recruitment of chondrogenic progenitor cells in a hyaluronate-fibrin hydrogel, which formed hyaline cartilage when cultured in a bovine cartilage explant model

Carbonyl detection by Western Blotting For the Western Blot, carbonyls were derivatized with 10 mM EZ-Link Alkoxyamine-PEG4-Biotin (Thermofisher) for 3H at RT

Refrigeration slows these reactions down dramatically, extending the peptide's useful life from a few days to several weeks or even months, depending on the concentration and handling

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