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Interestingly, anti-NTD-GST serum pool recognized the control peptide, Pep4 ( Figure 8C ), that contains the highly conserved RI (KLKQP sequence) present in CSP from all species of Plasmodium , with a titer (1:32,000) similar to that measured against the 6 repeats peptide (NANP24) of the repeat region when Fl-CSP was used as an immunogen

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Additionally, Massons trichrome staining was used to assess the total collagen level in the wound site, which serves as a main marker of the wound-healing process