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After 15 min, all rats received an injection of fentanyl (75 g/kg, IV)

The remaining fat becomes more resistant to mobilization

For sensitive assays, use freshly reconstituted material

FIGURE 1 3.2 Daphnetin attenuated oxidative stress of diabetes cognitive dysfunction rats As shown in Figure 2, STZ-treated caused oxidative stress in rat brain, which increased the MDA content and GSSH content, and reduced the levels of SOD, CAT, GSH, Gpx and TAC, while daphnetin-treated inhibited these changes in oxidative stress-related medicators, which indicated that daphnetin could mitigate oxidative stress of diabetes cognitive dysfunction rats

This can then be used to form a peptide (amide) bond to a second amino acid