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The absorbance for each condition was measured, and the percentage of cross-reactivity was calculated using the following equation, adapted for non-competitive sandwich immunoassays 53 : Cross-reactivity (%) = [(OD of cross-reactant analyte)/(OD of target analyte)] 100

the Schwartz patent references the use of the measured levels of constituents of the pathway as indicators of the need to administer the constituents of the SAM pathway, and references cysteine and glutathione as an indicator of dysfunction of the SAM pathway