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Initially, growth factors such as 20 ng/mL BMP2 and 30 ng/mL FGF4 were used as a control

The introduction of wortmannin was found to significantly reverse the expression of PI3K and AKT, as well as the anti-apoptotic and anti-inflammatory effects of Dihexa, resulting in a decrease in the number of neuronal cells present in the cortex and the levels of IL-10, and an increase in the levels of TNF-alpha and IL-1-beta [1]

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Male rats were used over female rats in order to eliminate any skewed data that may result from hormonal fluctuations related to the rats reproductive cycles