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After 24 h the colonies on the plates were counted and numbers expressed as CFU/ml

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This varies by surgery type, commute, anaesthesia recovery, and patient comfort

Materials and methods

Biotin-enhanced formulas Some ALA supplements include added biotin as a precaution, based on limited evidence primarily from animal studies suggesting high doses of ALA might interfere with biotin metabolism.57 While the real-world significance in humans remains unclear, manufacturers include biotin to help prevent any potential deficiency