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Cell lysates were separated via 412% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), following which the proteins were transferred to polyvinylidene difluoride (PVDF) membranes

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Internal changes during this phase include upregulation of collagen genes, increased growth factor production, and early accumulation of new extracellular matrix components

By preventing this, glutathione helps protect against signs of ageing and maintain radiance

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