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At study endpoints, mice were euthanized by carbon dioxide (CO) inhalation followed by cervical dislocation, in accordance with institutional animal care and use guidelines

The study protocol was approved by the Independent Bioethics Committee for Scientific Research at the Medical University of Gdansk (NKBBN/354 201/2017) and registered in the ClinicalTrials.gov Registry (NCT05120011)

Another paper reveals that these atmospheric jet streams circulate below each of the zones and belts, and reach all the way down to 1,900 miles

10.1086/669665 61 LissettC

The approach to the operation varies from patient to patient depending on your goals, skin elasticity and skin and muscle quality