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Standard or sample was transferred in triplicate to a 96-well plate and GSH was then derivitized by the addition of 10 mM 2,3-naphthalenedicarboxyaldehyde and the fluorescence at Ex/Em wavelength of 472/528 nm was quantified using a fluorescent plate reader (Synergy 2, BioTek)

They work differently, have distinct challenges, and are better suited to different goals

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It's essential to the production and replication of white blood cells known as lymphocytes, and it's particularly vital to the activity of specific lymphocytes called "natural killer" or NK cells

Antibodies Mouse momoclonal Anti-CD86 antibody, Abcam (Product # 130-122-1), Dilution 1:200