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After blocking in 1% skim milk in Tw-PBS for 1 h at room temperature, the membrane was incubated with SMA (dilution 1:2000

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Chemical synthesis method: usually involves protection and deprotection steps

Retatrutide a triple agonist of GLP-1, GIP, and glucagon receptors (half-life ~6 days), promoting insulin secretion, hepatic fat oxidation, appetite suppression, and energy expenditure