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There has many researches about increasing the production of l-cysteine, such as reduced the degradation of l-cysteine by disrupting the l-cysteine degradation genes [19] or overexpressed the l-cysteine synthetases to increase the l-cysteine concentration [20, 21]

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doi: 10.1016/j.molmet.2021.101206 54 JacobsenJChristoffersenB

Intracellular ROS detection Raw264.7 cells (30-40% confluence) seeded onto a confocal dish were stimulated with lipopolysaccharide (LPS

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After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with either TGF1 10ng/ml (Peprotech, Dollard des Ormeaux, Quebec, Canada), GHK 10 nM (Sigma, Markham, Ontario, Canada) or control media (1% FBS DMEM, penicillin, streptavidin) for a further 48 h