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Image-J was used for quantification by converting images into binary images via an intensity threshold and positive area was quantified 94

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Similar results were obtained for two other human albumin fusion designs, where either recombinant coagulation factor IX (FIX) 9 or angiotensin-converting enzyme 2 (ACE2) 13 was genetically fused to the N-terminal end of human albumin (Supplementary Fig