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The SH2 domain mediates tyrosine phosphorylation, primarily by JAK2, and subsequent homo- or heterodimerization of STAT5 proteins

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Cells were FBS starved for 6 hours with DMEM containing 0.1% BSA and incubated with KRH buffer (4 mM KH 2 PO 4 , 1 mM MgSO 4 , 1 mM CaCl 2 , 120 mM NaCl, 10 mM NaHCO 3 , 30 mM HEPES, pH 7.4) for 20 minutes

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