Mech Ageing Dev (2019) 177:3745
In vitro interaction assays were performed with equal amounts of GST or GST fusion proteins in affinity buffer (20 mM Hepes, 10 M ZnCl 2 , 0.1% Triton, 2 mM EDTA) supplemented with NaCl, 1 mM DTT, 0.1 mM PMSF and protease inhibitor cocktail (Sigma)
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Ultrasound of tendons and entheses can show thickening and power Doppler activity confirming active inflammation
Structurally optimized derivatives like CMC2.24 demonstrate enhanced efficacy, inhibiting tyrosinase activity in MNT-1 human melanoma cells at 525 M without cytotoxicity (Goenka et al., 2021)
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