Intracellular ROS are believed to be mainly produced by the mitochondria and NADPH oxidase (NOX) and can be associated with various physiological processes, such as proliferation, cell signaling, and oxygen homeostasis
The pore sizes are smaller in the higher percentage of agarose beads, so its useful to separate small globular proteins
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Statistical analysis was performed using a one-way analysis of variance and was analyzed further by the NewmanKeuls test for significant differences
If using controls, prepare control samples without GPx to serve as background controls