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The reaction was stopped by adding 3x SDS sample buffer and boiling at 95 C for 10 min before the samples were separated by SDS-PAGE and chain cleavage was analyzed by immunoblotting using a primary anti-SUMO2 antibody (Thermo, cat no

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In the original healthy-adult study of albumin-binding CJC-1295, single subcutaneous doses raised mean plasma GH 2- to 10-fold for six days or more and mean IGF-I 1.5- to 3-fold for nine to eleven days, with an estimated elimination half-life on the order of roughly six to eight days a pharmacokinetic profile measured in days , not minutes