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Cell Imaging/Staining RT-PCR Cell Proliferation/Viability Assay Cell Migration/Invasion Assay Cell Proliferation/Viability Assay All DNA/RNA Synthesis Isoforms More Biological Activity antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis