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For pathway reconstitution experiments, the codon-optimized BCCT gene (Aci1347) was cloned into plasmid pGEX-6P-1- cntAB using the In-Fusion cloning system (Takara) yielding construct pGEX-6P-1- cntAB-bcct

doi: 10.15252/embr.201745130 23

Matuszczak Y, et al

Epigenetic modifications, such as increased methylation and expression of the pseudogene PTENP1, occur with age, possibly as a protective mechanism against malignancy [43]

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That change, although it seems small, places ALCAR in an interesting spot within the carnitine world