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Protein extracts (1575 g) were boiled at 95 C for 5 min in loading buffer (100 mM Tris pH 6.8, 10% glycerol, 4% sodium dodecyl sulfate (SDS), 0.2% bromophenol blue and 2 mM -mercaptoethanol) and subjected to 812% SDS polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride (PVDF) membranes

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Clinical Trials Cagrilintide is a long-acting amylin analogue that has demonstrated significant potential in weight management research, particularly when combined with GLP-1 receptor agonists

21 Other tests include magnetic resonance elastography and various other scoring systems (Table 5)

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