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Conversely, overexpression of HNF4 in HNF4-negative SNU449 cells strongly induced the luciferase activities from reporters containing the consensus (WT) HNF4 binding sites of MAT1A , BHMT , or CBS promoters, but this induction was significantly reduced when the binding sites were mutated (Mut) (Supplementary Fig

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Once outside the cell, GSH degradation occurs exclusively in the extracellular space and only on the surface of cells that express the ectoenzyme -glutamyl transferase

Sample preparations for plasma, urine, hair, and tissue All samples were processed with a standard operating procedure.150 L aliquots of thawed plasma or urine were mixed with three internal standards (IS) [20 L of d4-alanine (Sigma, USA, 10 mM), d5-phenylalanine (Sigma, USA, 10 mM), and d5-tryptophan (Sigma, USA, 10 mM)]

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