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Ferroptosis, modulated by pathways such as autophagy, is shown to be impaired by Fer-1 treatment, which alleviates SVV-induced degradation of GPX4 and inhibits LC3-II conversion (Fig

In vitro interaction assays were performed with equal amounts of GST or GST fusion proteins in affinity buffer (20 mM Hepes, 10 M ZnCl 2 , 0.1% Triton, 2 mM EDTA) supplemented with NaCl, 1 mM DTT, 0.1 mM PMSF and protease inhibitor cocktail (Sigma)

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