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Step 1: Prepare Sterile Materials and Calculate Reconstitution Volume Before opening any vial, assemble all materials in a clean workspacepreferably a dedicated research area free from airborne contaminants

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Cysteine rich with EGF like domains 2 (CRELD2, also named MESFATIN), whose gene was mesenteric adipose tissue specifically expressed and upregulated by CR in rat chips, was selected and verified as novel adipokine with proving its expression and secretion in in vivo mouse, rat and human and in vitro adipose tissue and adipocyte

95% CI 1.06- 1.16) (211)

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