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The 1% concentration range used in most published clinical trials sits at the level demonstrated to produce dermal effects while maintaining a formulation viscosity optimized for absorption

Cloning, heterologous expression and protein purification All GS-like genes analysed were cloned (without their predicted signal peptide if appropriate (SignalP v4.0)) into the pOPINS3C vector [66] in frame with an N-terminal poly-Histidine tag, a SUMO chaperone to promote protein solubility, and a 3C protease cleavage site (S5 Table)

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Potential dermatologic adverse events in this population remain underexplored, the authors wrote

There is also an increasing burden of HF: in a study of 4 million patient records in the UK between 2002 and 2014, there was an estimated increase of 12% in individuals with newly diagnosed HF and a 23% estimated increase in the number of prevalent HF cases [36]