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Glutathione is one of the bodys most important endogenous antioxidant compounds and serves as a cofactor in natural detoxification processes

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A GST-tagged protein is immobilized on glutathione agarose beads and used to pull down interacting proteins from a complex mixture, such as cell lysate, or using purified recombinant proteins

Oxidative stress responses clarified the functioning of central principal switches like nuclear factor (NF)-B (NFB) or the Kelch-like ECH-associated protein 1 (Keap1)-Nuclear factor erythroid 2-related factor2 (Nrf2)-antioxidant response element (ARE) redox regulator pathway (Sies, 2015

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This gene modulation effect extends to pathways related to wound response, growth factors, and anti-fibrotic mechanisms, making it of growing interest in regenerative biology studies