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The cell suspension was passed through a 70 m nylon mesh, centrifuged (300 g for 7 min at room temperature, 2021 C) and chondrocytes were seeded in DMEM (Gibco) containing 5.5 mM glucose (Sigma-Aldrich), 2 mM glutamine (Gibco), 1 mM sodium pyruvate (Gibco) and supplemented with 100 units per ml penicillin, 50 g ml 1 streptomycin and 10% FBS (hereafter referred to as growth medium) at a density of 18 10 4 cells per cm

Emulsify with water until it turns milky, then rinse off with lukewarm water 4