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For ELISA, liver tissues were sonicated in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100 and protease inhibitor cocktail (EDTA-free Complete Mini, Roche)

In working with international victims, this responsibility falls primarily to an assigned case manager, often funded completely or partly with Federal funding

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Furthermore, ARP also had some effects on reducing ROS levels and increasing ATP levels, thereby significantly improving diet-induced OS (Chen et al., 2021)

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