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Briefly, Cas9-expressing cells were transduced with a lentiviral vector consisting of blue fluorescent protein (BFP) and green fluorescent protein (GFP) expression cassettes as indicated, together with a sgRNA targeting GFP

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Immunohistochemistry (hair cell viability)Fixed and dissected cochleae are incubated in PBS-T with 10% normal goat serum with a phalloidin primary antibody overnight at 4 C

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7 Energy and motivation: TRT stimulates neurotransmitterslike dopamine, epinephrine, and norepinephrinein the brain, which improves mood, focus, and motivation, says Lee