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The supernatants were analyzed using the Glucagon-Like Peptide-1 (Active) ELISA Kit (Millipore Sigma, Saint Louis, MO, USA) 28

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You should contact your GP or healthcare provider if you experience: Persistent extreme cold intolerance that significantly affects your daily activities or quality of life Cold sensitivity accompanied by other symptoms of hypothyroidism , such as unexplained weight gain (despite continued Wegovy use), severe fatigue, constipation, dry skin, hair loss, or depression Shivering or inability to warm up even in heated environments Changes in heart rate, particularly a notably slow pulse (bradycardia) Swelling in the neck or difficulty swallowing Any new or worsening symptoms that concern you Your GP may wish to perform thyroid function tests (typically measuring TSH and possibly free T4) to rule out hypothyroidism if symptoms suggest this condition