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Cells were lysed as previously described (Mukanganyama et al., Preparation of Alkylated GSTs Using iodo-APAP or IAM Reductive alkylation of human GSTA1, M1, M2, or P1 (1.83.6 nmol) was performed in 100 mM ammonium bicarbonate (ABC, pH 8.5) using DTT (250 nmol
10.1111/acel.13034 56 PassosJ
A notable reduction in MDA levels was observed upon treatment with MWCNTs, where the lowest content was recorded at 8 mg L -1 i.e
Likewise, the origin of the glutathione that accumulates in this compartment remains unclear, as does whether external factors, such as glutathione-rich diets or direct intake of this compound as an antioxidant supplement, might influence its levels in the tumour microenvironment